https://doi.org/10.25678/000DJV

Data for: Physiological and transcriptomic responses in brown trout, Salmo trutta, to multiple stressors: pesticide mixtures, elevated water temperature and the proliferative kidney disease

Contamination with pesticides, rising water temperatures and pathogen pressure represent a multiple stressor scenario relevant to surface water ecosystems globally. This study investigated the combination of three environmental stressors on juvenile brown trout, Salmo trutta. Fish acclimatised to either 12 or 15°C, were exposed for 14 days to one (at 12°C) or two (at 15°C) sublethal concentrations of a pesticide mixture (fluopyram, epoxiconazole, diuron, chlorpyrifos, λ-cyhalothrin), then were maintained in clean water for three months, with half exposed to T. bryosalmonae, the causative agent of proliferative kidney disease (PKD). Pesticide exposure alone neither caused mortality nor changes in growth, hematocrit, or organ indices. However, the transcriptome (RNA-Seq) and gene expression (RT-qPCR) in brain and liver were significantly altered. No interactions between temperature and pesticide exposure were observed on apical, physiological, or qPCR endpoints and susceptibility to PKD was not affected by pesticide exposure. However, transcriptomic analysis revealed that the number of differentially expressed genes (DEGs) induced by pesticides was greater at 15°C. Moreover, the number of DEGs affected by temperature in the brain was strongly reduced in the presence of pesticides. In addition, fish exhibited decreased basal oxygen consumption 2.5 months after exposure to the higher pesticide concentration at 15°C suggesting potential metabolic trade-offs. Overall, our findings emphasize the need for ecotoxicological studies to incorporate multi-stressor scenarios and the importance of considering sublethal effects in understanding the response of fish populations to pesticide contamination in a changing climate.

Data and Resources

Citation

This Data Package

Voisin, A.-S., Rehberger, K., Fasel, M., Beauvais, R., Segner, H., & Werner, I. (2025). Data for: Physiological and transcriptomic responses in brown trout, Salmo trutta, to multiple stressors: pesticide mixtures, elevated water temperature and the proliferative kidney disease (Version 1.0). Eawag: Swiss Federal Institute of Aquatic Science and Technology. https://doi.org/10.25678/000DJV

The associated article

Voisin, A.-S., Rehberger, K., Fasel, M., Beauvais, R., Segner, H., & Werner, I. (2025). Physiological and transcriptomic responses in brown trout, Salmo trutta, to multiple stressors: Pesticide mixtures, elevated water temperature and the proliferative kidney disease. Science of The Total Environment, 986, 179727. https://doi.org/10.1016/j.scitotenv.2025.179727

Metadata

  Publication Data Package for:
Open Data Open Data
Author
  • Voisin, Anne-Sophie
  • Rehberger, Kristina
  • Fasel, Melanie
  • Beauvais, Rébecca
  • Segner, Helmut
  • Werner, Ingeborg
Keywords Multiple stressors,Pesticides,Temperature,Proliferative kidney disease,Transcriptomics,Salmo trutta
Variables
  • gene_abundance
  • length
  • mortality
  • weight
Substances (scientific names)
  • chlorpyrifos (InChI=1S/C9H11Cl3NO3PS/c1-3-14-17(18,15-4-2)16-9-7(11)5-6(10)8(12)13-9/h5H,3-4H2,1-2H3)
  • diuron (InChI=1S/C9H10Cl2N2O/c1-13(2)9(14)12-6-3-4-7(10)8(11)5-6/h3-5H,1-2H3,(H,12,14))
  • epoxiconazole (InChI=1S/C17H13ClFN3O/c18-15-4-2-1-3-14(15)16-17(23-16,9-22-11-20-10-21-22)12-5-7-13(19)8-6-12/h1-8,10-11,16H,9H2/t16-,17-/m1/s1)
  • fluopyram (InChI=1S/C16H11ClF6N2O/c17-12-7-9(15(18,19)20)8-25-13(12)5-6-24-14(26)10-3-1-2-4-11(10)16(21,22)23/h1-4,7-8H,5-6H2,(H,24,26))
  • lambda-cyhalothrin (InChI=1S/C23H19ClF3NO3/c1-22(2)17(12-19(24)23(25,26)27)20(22)21(29)31-18(13-28)14-7-6-10-16(11-14)30-15-8-4-3-5-9-15/h3-12,17-18,20H,1-2H3/b19-12-/t17-,18+,20-/m1/s1)
Substances (generic terms)
  • fungicide
  • herbicide
  • insecticide
  • pesticides
  • plant protection products
Taxa (scientific names)
  • Salmo trutta
Organisms (generic terms)
  • fish
Systems
  • laboratory
Timerange
  • 2019
Review Level none
Curator Voisin, Anne-Sophie
Contact Ferrari, Benoit <Benoit.Ferrari@centreecotox.ch>
DOI 10.25678/000DJV