Data for: Micropollutant Biotransformation and Bioaccumulation in Natural Stream Biofilms

Micropollutants are ubiquitously found in natural surface waters and pose a potential risk to aquatic organisms. Stream biofilms, consisting of bacteria, algae and other microorganisms potentially contribute to bioremediating aquatic environments by biotransforming xenobiotic substances. When investigating the potential of stream biofilms to remove micropollutants from the water column, it is important to distinguish between different fate processes, such as biotransformation, passive sorption and active bioaccumulation. However, due to the complex nature of the biofilm community and its extracellular matrix, this task is often difficult. In this study, we combined biotransformation experiments involving natural stream biofilms collected up- and downstream of wastewater treatment plant outfalls with the QuEChERS extraction method to distinguish between the different fate processes. The QuEChERS extraction proved to be a suitable method for a broad range of micropollutants (> 80% of the investigated compounds). We found that 31 out of 63 compounds were biotransformed by the biofilms, with the majority being substitution-type biotransformations, and that downstream biofilms have an increased biotransformation potential towards specific wastewater-relevant micropollutants. Overall, using the experimental and analytical strategy developed, stream biofilms were demonstrated to have a broad inherent micropollutant biotransformation potential, and to thus contribute to bioremediation and improving ecosystem health.

Dataset extent

Data and Resources


This Data Package

Desiante, W. L., Minas, N. S., & Fenner, K. (2020). Data for: Micropollutant Biotransformation and Bioaccumulation in Natural Stream Biofilms (Version 1.0) [Data set]. Eawag: Swiss Federal Institute of Aquatic Science and Technology.

The associated article

Desiante, W. L., Minas, N. S., & Fenner, K. (2021). Micropollutant biotransformation and bioaccumulation in natural stream biofilms. Water Research, 193, 116846.


Open Data Open Data
Long-term data Long-term data
  • Desiante, Werner Leonardo
  • Minas, Nora Sophia
  • Fenner, Kathrin
Keywords Micropollutants,Stream biofilms,Biotransformation,QuEChERS extraction,Bioremediation,pesticides,pharmaceuticals,artificial sweeteners,periphyton,sorption,Organic contaminants,Microbial ecotoxicology,Biofilm extraction
  • concentration
  • flow_cytometric_cell_counts
  • PAR
Substances (scientific names)
  • 2-6-Dichlorbenzamid
  • 5-Methyl-Benzotriazol
  • Acesulfam
  • Acetamiprid
  • Amisulpride
  • Atenolol
  • Azoxystrobin
  • Benzotriazol
  • Bromoxynil
  • Candesartan
  • Capecitabin
  • Carbamazepin
  • Carbendazim
  • Cetirizin
  • Cilastatin
  • Citalopram
  • Clofibric acid
  • Coffein
  • Cyclamat
  • Diclofenac
  • Dimethenamid
  • Eprosartan
  • Ethofumesate
  • Fenhexamid
  • Fenofibrate
  • Fipronil
  • Furosemide
  • Gabapentin
  • Gemfibrozil
  • Hydrochlorothiazid
  • Imidacloprid
  • Indomethazin
  • Iprovalicarb
  • Isoproturon
  • Ketoprofen
  • Kresoxim-methyl
  • Lamotrigin
  • Levamisol
  • Levetiracetam
  • Lidocaine
  • Mecoprop
  • Metoprolol
  • Mianserin
  • N-N-diethyl-3-methylbenzamid (DEET)
  • Oxcarbazepin
  • Paracetamol (3-Acetamidophenol)
  • Pheniramine
  • Propachlor
  • Propanolol
  • Pyraclostrobin
  • Ranitidine
  • Rufinamid
  • Saccharin
  • Sulfadiazin
  • Sulfamethazin
  • Sulfamethoxazol
  • Sulfapyridin
  • Sulfathiazol
  • Thiacloprid
  • Trifloxystrobin
  • Trinexapac-ethyl
  • Valsartan
  • Venlafaxin
Substances (generic terms)
  • Artificial sweeteners
  • Micopollutants
  • Pesticides
  • Pharmaceuticals
Organisms (generic terms)
  • periphyton
  • Stream biofilms
  • batch experiments
  • river
  • stream
  • 2017-04-20 TO 2017-05-22
  • 2018-09-11 TO 2018-10-08
Geographic Name(s)
  • Ron (Hochdorf)
  • Rot Zufluss (Buttisholz)
Review Level domain specific
Curator Desiante, Werner
DOI 10.25678/0002J8