https://doi.org/10.25678/00058M

Data for: Wastewater treatment plant resistomes are shaped by bacterial composition, genetic exchange, and upregulated expression in the effluent microbiomes

Wastewater treatment plants (WWTPs) are implicated as hotspots for the dissemination of antibacterial resistance into the environment. However, the in situ processes governing removal, persistence, and evolution of resistance genes during wastewater treatment remain poorly understood. Here, we used quantitative metagenomic and metatranscriptomic approaches to achieve a broad-spectrum view of the flow and expression of genes related to antibacterial resistance to over 20 classes of antibiotics, 65 biocides, and 22 metals. All compartments of 12 WWTPs share persistent resistance genes with detectable transcriptional activities that were comparatively higher in the secondary effluent, where mobility genes also show higher relative abundance and expression ratios. The richness and abundance of resistance genes vary greatly across metagenomes from different treatment compartments, and their relative and absolute abundances correlate with bacterial community composition and biomass concentration. No strong drivers of resistome composition could be identified among the chemical stressors analyzed, although the sub-inhibitory concentration (hundreds of ng/L) of macrolide antibiotics in wastewater correlates with macrolide and vancomycin resistance genes. Contig-based analysis shows considerable co-localization between resistance and mobility genes and implies a history of substantial horizontal resistance transfer involving human bacterial pathogens. Based on these findings, we propose future inclusion of mobility incidence (M%) and host pathogenicity of antibiotic resistance genes in their quantitative health risk ranking models with an ultimate goal to assess the biological significance of wastewater resistomes with regard to disease control in humans or domestic livestock.

Data and Resources

Citation

This Data Package

Ju, F., Beck, K., Yin, X., Maccagnan, A., McArdell, C. S., Singer, H. P., et al. (2019). Data for: Wastewater treatment plant resistomes are shaped by bacterial composition, genetic exchange, and upregulated expression in the effluent microbiomes (Version 1.0). Eawag: Swiss Federal Institute of Aquatic Science and Technology. https://doi.org/10.25678/00058M
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The associated article

Ju, F., Beck, K., Yin, X., Maccagnan, A., McArdell, C. S., Singer, H. P., et al. (2018). Wastewater treatment plant resistomes are shaped by bacterial composition, genetic exchange, and upregulated expression in the effluent microbiomes. The ISME Journal, 13(2), 346–360. https://doi.org/10.1038/s41396-018-0277-8
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Metadata

  Publication Data Package for:
Open Data Open Data
Author
  • Ju, Feng
  • Beck, Karin
  • Yin, Xiaole
  • Maccagnan, Andreas
  • McArdell, Christa S.
  • Singer, Heinz P.
  • Johnson, David R.
  • Zhang, Tong
  • Bürgmann, Helmut
Keywords wastewater,antibiotic resisance,metagenomics,metatranscriptomics
Variables
  • concentration
  • count
  • pH
Substances (scientific names)
  • Azithromycin (MQTOSJVFKKJCRP-BICOPXKESA-N)
  • Ciprofloxacin (MYSWGUAQZAJSOK-UHFFFAOYSA-N)
  • Clarithromycin (AGOYDEPGAOXOCK-KCBOHYOISA-N)
  • Clindamycin (KDLRVYVGXIQJDK-AWPVFWJPSA-N)
  • Erythromycin (ULGZDMOVFRHVEP-RWJQBGPGSA-N)
  • Levofloxacin (GSDSWSVVBLHKDQ-JTQLQIEISA-N)
  • Metformin (XZWYZXLIPXDOLR-UHFFFAOYSA-N)
  • Metronidazole (VAOCPAMSLUNLGC-UHFFFAOYSA-N)
  • Moxifloxacin (FABPRXSRWADJSP-MEDUHNTESA-N)
  • N4-Acetylsulfamethoxazole (GXPIUNZCALHVBA-UHFFFAOYSA-N)
  • Norfloxacin (OGJPXUAPXNRGGI-UHFFFAOYSA-N)
  • Ofloxacin (GSDSWSVVBLHKDQ-UHFFFAOYSA-N)
  • Roxithromycin (RXZBMPWDPOLZGW-XMRMVWPWSA-N)
  • Sulfadiazine (SEEPANYCNGTZFQ-UHFFFAOYSA-N)
  • Sulfamethoxazole ( JLKIGFTWXXRPMT-UHFFFAOYSA-N)
  • Sulfapyridine (GECHUMIMRBOMGK-UHFFFAOYSA-N)
  • Triclosan (XEFQLINVKFYRCS-UHFFFAOYSA-N)
  • Trimethoprim (IEDVJHCEMCRBQM-UHFFFAOYSA-N)
  • Vancomycin (MYPYJXKWCTUITO-LYRMYLQWSA-N)
Substances (generic terms)
  • antibiotics
  • nutirents
  • organic matter
  • pharmaceuticals
Taxa (scientific names)
  • Bacteria
Organisms (generic terms)
  • Bacteria
  • Microorganisms
Systems
  • actiated sludge (denitrification stage)
  • activated sludge (nitrification stage)
  • wastewater treatment plant
  • WWTP
  • WWTP effluent
  • WWTP influent
Timerange
  • 2016-03 TO 2016-04
Geographic Name(s)
  • ARA Altdorf
  • ARA Altenrhein
  • ARA Bachwis, Fällanden
  • ARA Bern
  • ARA Fribourg
  • ARA Laufäcker, Turgi
  • ARA Oberes Wiggertal
  • ARA Real, Emmen
  • ARA Stadtwerk Winterthur
  • ARA Surental, Triengen
  • ARA Thunersee
  • ARA Zürich Werdhölzli
  • Switzerland
Review Level general
Curator Buergmann, Helmut
Contact Helmut.Buergmann@eawag.ch
DOI 10.25678/00058M