https://doi.org/10.25678/0008EB

Data for: Timing of antibiotic administration determines the spread of plasmid-encoded antibiotic resistance during microbial range expansion

Plasmids are the main vector by which antibiotic resistance is transferred between bacterial cells within surface-associated communities. In this study, we ask whether there is an optimal time to administer antibiotics to minimize plasmid spread in new bacterial genotypes during community expansion across surfaces. We address this question using consortia of Pseudomonas stutzeri strains, where one is an antibiotic resistance-encoding plasmid donor and the other a potential recipient. We allowed the strains to co-expand across a surface and administered antibiotics at different times. We find that plasmid transfer and transconjugant proliferation have unimodal relationships with the timing of antibiotic administration, where they reach maxima at intermediate times. These unimodal relationships result from the interplay between the probabilities of plasmid transfer and loss. Our study provides mechanistic insights into the transfer and proliferation of antibiotic resistance-encoding plasmids within microbial communities and identifies the timing of antibiotic administration as an important determinant.

Data and Resources

Citation

This Data Package

Johnson, D., Ma, Y., & Ramoneda, J. (2023). Data for: Timing of antibiotic administration determines the spread of plasmid-encoded antibiotic resistance during microbial range expansion (Version 1.0). Eawag: Swiss Federal Institute of Aquatic Science and Technology. https://doi.org/10.25678/0008EB
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The associated article

Ma, Y., Ramoneda, J., & Johnson, D. R. (2023). Timing of antibiotic administration determines the spread of plasmid-encoded antibiotic resistance during microbial range expansion. Nature Communications, 14(1). https://doi.org/10.1038/s41467-023-39354-z
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Metadata

  Publication Data Package for:
Open Data Open Data
Long-term data Long-term data
Author
  • Johnson, David
  • Ma, Yinyin
  • Ramoneda, Josep
Keywords biofilms,antibiotic resistance,bacteria,microbial communities
Variables
  • bacteria_abundance
  • count
  • fluorescence
Taxa (scientific names)
  • Pseudomonas stutzeri
Organisms (generic terms)
  • bacteria
Systems
  • lab
Timerange
  • 2019 TO 2022
Review Level domain specific
Curator Johnson, David
Contact Johnson, David <David.Johnson@eawag.ch>
DOI 10.25678/0008EB