https://doi.org/10.25678/0007GJ

Data for: Fungal hyphae regulate bacterial diversity and plasmid-mediated functional novelty during range expansion

The amount of bacterial diversity present on many surfaces is enormous, yet how these levels of diversity persist in the face of the purifying processes that occur as bacterial communities expand across space (referred to here as range expansion) remains enigmatic. We shed light on this apparent paradox by providing mechanistic evidence for a strong role of fungal hyphae-mediated dispersal on regulating bacterial diversity during range expansion. Using pairs of fluorescently labelled bacterial strains and a hyphae-forming fungal strain that expand together across a nutrient-amended surface, we show that a hyphal network increases the spatial intermixing and extent of range expansion of the bacterial strains. This is true regardless of the type of interaction (competition or resource cross-feeding) imposed between the bacterial strains. We further show that the underlying cause is that flagellar motility drives bacterial dispersal along the hyphal network, which counteracts the purifying effects of ecological drift at the expansion frontier. We finally demonstrate that hyphae-mediated spatial intermixing increases the conjugation-mediated spread of plasmid-encoded antibiotic resistance. In conclusion, fungal hyphae are important regulators of bacterial diversity and promote plasmid-mediated functional novelty during range expansion in an interaction-independent manner.

Data and Resources

Citation

This Data Package

Johnson, D., Ruan, C., Ramoneda, J., Gogia, G., & Wang, G. (2022). Data for: Fungal hyphae regulate bacterial diversity and plasmid-mediated functional novelty during range expansion (Version 1.0) [Data set]. Eawag: Swiss Federal Institute of Aquatic Science and Technology. https://doi.org/10.25678/0007GJ

The associated article

Ruan, C., Ramoneda, J., Gogia, G., Wang, G., & Johnson, D. R. (2022). Fungal hyphae regulate bacterial diversity and plasmid-mediated functional novelty during range expansion. Current Biology. https://doi.org/10.1016/j.cub.2022.11.009

Metadata

Open Data Open Data
Long-term data Long-term data
Author
  • Johnson, David
  • Ruan, Chujin
  • Ramoneda, Josep
  • Gogia, Guram
  • Wang, Gang
Keywords Range expansion,microbial dispersal,biofilms,fungal hyphae,bacterial diversity,bacterial motility,plasmid conjugation,horizontal gene transfer,antibiotic resistance
Variables
  • count
  • flow_cytometric_cell_counts
  • fluorescence
  • length
  • total_cell_concentration
Substances (scientific names)
  • None
Substances (generic terms)
  • None
Taxa (scientific names)
  • Penecillium sp.
  • Pseudomonas aeruginosa
  • Pseudomonas stutzeri
Organisms (generic terms)
  • bacteria
  • fungi
Systems
  • lab
Timerange
  • 2020-01 TO 2022-01
Review Level general
Curator Johnson, David
Contact Johnson, David <David.Johnson@eawag.ch>
DOI 10.25678/0007GJ