https://doi.org/10.25678/00054G

Data for: Wastewater bypass is a major temporary point-source of antibiotic resistance genes and multi-resistance risk factors in a Swiss river

Untreated combined sewage (bypass) is often discharged by wastewater treatment plants to receiving rivers during stormwater events, where it may contribute to increased levels of antibiotic resistance genes (ARGs) and multi-resistance risk factors (multi-resistant bacteria and multi-resistance genomic determinants (MGDs)) in the receiving water. Other contamination sources, such as soil runoff and resuspended river sediment could also play a role during stormwater events. Here we report on stormwater event-based sampling campaigns to determine temporal dynamics of ARGs and multi-resistance risk factors in bypass, treated effluent, and the receiving river, as well as complimentary data on catchment soils and surface sediments. Both indicator ARGs (qPCR) and resistome (ARG profiles revealed by metagenomics) indicated bypass as the main contributor to the increased levels of ARGs in the river during stormwater events. Furthermore, we showed for the first time that the risk of exposure to bypass-borne multi-resistance risk factors increase under stormwater events and that many of these MGDs were plasmid associated and thus potentially mobile. In addition, elevated resistance risk factors persisted for some time (up to 22 hours) in the receiving water after stormwater events, likely due to inputs from distributed overflows in the catchment. This indicates temporal dynamics should be considered when interpreting the risks of exposure to resistance from event-based contamination. We propose that reducing bypass from wastewater treatment plants may be an important intervention option for reducing dissemination of antibiotic resistance.

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Lee, J., Beck, K., & Bürgmann, H. (2021). Data for: Wastewater bypass is a major temporary point-source of antibiotic resistance genes and multi-resistance risk factors in a Swiss river (Version 1.0) [Data set]. Eawag: Swiss Federal Institute of Aquatic Science and Technology. https://doi.org/10.25678/00054G

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The associated article

Lee, J., Beck, K., & Bürgmann, H. (2021). Wastewater bypass is a major temporary point-source of antibiotic resistance genes and multi-resistance risk factors in a Swiss river. Water Research, 117827. https://doi.org/10.1016/j.watres.2021.117827

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Author	Lee, Jangwoo Beck, Karin Bürgmann, Helmut
Keywords	Antimicrobial Resistance,Stormwater Events,Wastewater Bypass,Metagenomics,River,antibiotic resistance genes
Variables	bacteria_abundance electric_conductivity gene_abundance precipitation
Organisms (generic terms)	(cultivable) bacteria
Systems	bypass river sediment soil WWTP
Timerange	2019-06 TO 2019-09
Geographic Name(s)	River Murg, Switzerland WWTP Münchwilen, Thurgau, Switzerland
Review Level	domain specific
Curator	Lee, Jangwoo
Contact	jangwoo.lee@eawag.ch
DOI	10.25678/00054G